Multilayer dynamic light scattering in the analysis of the size and concentration of liposomes


Modern drug delivery systems include the use of liposomes, which means that their characteristics must be fully described for the purpose of producing the specifications and releasing them into batches.

Such a characterization process involves assessing the size of the liposomes and the particle size distribution.

© Tomasz Swierczynski /

Dynamic light scattering (DLS) is a common technique for this type of study, in addition to the recent tracking of nanoparticles (NTA), which assesses the size and concentration of liposomes. In this article, the use of multi-angle DLS (MADLS®) for such measurements at different concentrations is described using Zetasizer Ultra versus NTA performed on the same sample.

In this experiment, the liposomes were measured at 25 ° C five times with the Zetasizer Ultra using the particle concentration parameter and five times with the NanoSight NS300 with five video recordings lasting 60 seconds, together with the use of a spray pump and a laser at 405 nm.

Multi-angle Dynamic Lighting with Zetasizer Ultra – How Does It Work?

In general, MADLS is a good way to measure the concentration of particles and Zetasizer Ultra is used to measure the size and concentration of liposomes, indicating good consistency with the results achieved by NTA in the overlap area.

The combination of these two techniques is so complementary as NTA gives the necessary precision at lower sample concentrations and more detail on the size distribution, while MADLS allows the measurement of a large volume of sample concentrations along with the size distribution at a speed and without the need for a sample preparation.

About Malvern Panalytical

Malvern Panalytical offers materials and technology for biophysical characterization and expertise, which enables scientists and engineers to understand and control the properties of disperse systems.

These systems range from proteins and polymers in suspensions of solutions, particles and nanoparticles, and emulsions to sprays and aerosols, industrial bulk and highly concentrated sludges.

Used at all stages of research, development and production, Malvern Panalytical materials characterization materials provide critical information that helps accelerate research and product development, improve and maintain product quality, and optimize process efficiency.

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